Cytek Aurora

Cytometers

Consult us to select the device best suited to your application.

The Aurora cytometer is a spectral analyzer with the unique ability to measure all the emission spectra of fluorochromes or fluorescent probes excited by all the lasers installed on the instrument. Our Aurora is equipped with four lasers (UV/355, Violet/405, Blue/488 and Red/640 nm excitation wavelengths), three scattering channels (FSC and SSC on blue and violet lasers) and 54 fluorescence channels. Capturing the full spectrum enables new deconvolution algorithms to be used for subsequent data analysis. Until now, up to 40 different fluorochromes could be resolved simultaneously in a single panel, even when several markers are co-expressed on the same cell. The new SpectroFlo™ software offers intuitive working, from quality control to data analysis, with technological tools that simplify the execution of any application.

Key points:

  • 54 detection channels across all emission spectra
  • Over 40 fluorochromes simultaneously, including those with emission spectra very close to each other.
  • Redefined sensitivity thanks to state-of-the-art optics and low-noise electronics.
  • No need to change optical filters for any fluorochrome. Use any commercially available laser-excitable fluorescent molecule.
  • Automatic micro-sampling system (plate loader) enables acquisition of 96-well plates (U-bottom, V-bottom and flat-bottom) with ease of use, low carry-over and minimal dead volume. Unanalyzed sample recovery and temperature controller.
  • A powerful, high-value system accessible to a wide range of users
  • Absolute counting
  • 3 acquisition speeds: low (15µl/min), medium (30µl/min) and high (60µl/min) and 100µl/min in plate mode
  • Possibility of autofluorescence extraction to improve data resolution.

Spectral signature of APC obtained with the 4L UV16, V16, B14, R8 configuration

4L UV16-V16-B14-R8 Configuration

Resources :

  • Cytek® Full spectrum viewer : tool that lets you compare fluorescent molecule signatures to help you design your panel for Cytek Aurora and Cytek Northern Lights.
  • Cytek Cloud : new digital ecosystem that supports spectral flow cytometry research, from panel design to data acquisition. It includes two integrated online tools, Panel Builder and Experiment Builder, to streamline your experiments on Northern Lights™, Aurora and Aurora CS… systems.